Main Article Content

Authors

Scenedesmus sp. & Chlorella sp., are two isolated microalgae from hot springs located in Norte de Santander, which are studied in order to explore their biotechnological potential. The present work aimed to design a protocol by evaluating two factors, the effect of light radiation stress and incubation time by staggering the culture from box to tube with 20 mL of medium and finally in a 200 mL reactor. From this, in order to obtain a higher production of carotenoids of industrial interest, therefore, a non-factorial central composite surface design was used; As results, it was determined that in Scenedesmus sp microalgae the two factors influence carotenoid deposition and for Chlorella sp the incubation time does not influence and the photoperiod is fundamental in the deposition of these metabolites, on the other hand the program in which evaluated the STATISTICA 7.0 design generates its respective equation from which the optimal incubation time and photoperiod for the studied microalgae were determined.

1.
Martínez JBG, Machuca-Martinez F, Cardenas-Gutierrez IY. Protocol for the maintenance of strains and escalation in the production of microalgae of industrial interest. inycomp [Internet]. 2021 Jan. 15 [cited 2024 Nov. 21];23(1):e10673. Available from: https://revistaingenieria.univalle.edu.co/index.php/ingenieria_y_competitividad/article/view/10673

(1) Cezare-Gomes EA, Mejia-da-Silva L del C, Pérez-Mora LS, Matsudo MC, Ferreira-Camargo LS, Singh AK, et al. Potential of Microalgae Carotenoids for Industrial Application. Appl Biochem Biotechnol. 2019;188:602–634. https://doi.org/10.1007/s12010-018-02945-4.

(2) Torregrosa-Crespo J, Montero, Z., Fuentes J, Reig García-Galbis M, Garbayo I, Vílchez C, Martínez-Espinosa R. Exploring the Valuable Carotenoids for the Large-Scale Production by Marine Microorganism. Mar Drugs. 2018;16(6):203.https://doi.org/10.3390/md16060203.

(3) Novovesk L, Ross M, Stanley M, Pradelles R, Wasiolek V, Sassi J. Microalgal Carotenoids : A Review of Production , Current Markets , Regulations , and Future Direction. Mar Drugs. 2019;17(11):640. https://doi.org/10.3390/md17110640.

(4) Gong M, Bassi A. Carotenoids from microalgae: A review of recent developments. Biotechnol Adv. 2016;34(8):1396–412. https://doi.org/10.1016/j.biotechadv.2016.10.005.

(5) Borowitzka M. Commercial-Scale Production of Microalgae for Bioproducts. In: La Barre S, Bates S, editors. Blue Biotechnology: Production and Use of Marine Molecules. rev. Weinheim, Germany: WILEY-VCH Verlag GmbH & Co; 2018. p. 51–2.

(6) Alvensleben N, Heimann K. The Potential of Microalgae for Biotechnology: A Focus on Carotenoids. In: La Barre S, Bates S, editors. Blue Biotechnology: Production and Use of Marine Molecules. rev. Weinheim, Germany: WILEY-VCH Verlag GmbH & Co; 2018. p. 131–2.

(7) Pourkarimi S, Hallajisani, A Alizadehdakhel A, Nouralishahi A. Biocatalysis and Agricultural Biotechnology Factors affecting production of beta-carotene from Dunaliella salina microalgae. Biocatal Agric Biotechnol. 2020;29:101771. https://doi.org/10.1016/j.bcab.2020.101771.

(8) Andersen R, Berges J, Harrison P, Watanabe M. Appendix A—Recipes for Freshwater and Seawater Media. In: Andersen R, editor. Algal Culturing Techniques. Burlington, MA: Elsevier Academic Press; 2005. p. 429–538.

(9) Statsoft I. STATISTICA (data analysis software system) [Internet]. 2004. Available from: https://www.statsoft.de/en/statistica/statistica-software.

(10) Moheimani N, Borowitzka M, Isdepsky A, Fon-Sing S. Standard methods for measuring growth of algae and their composition. In: Borowitzka M, Moheimani N, editors. Algae for biofuels and energy. Springer, Dordrecht; 2013. p. 265–284.

(11) Přibyla P, Cepáka V, Kaštánekb P, Zachlederc V. Elevated production of carotenoids by a new isolate of Scenedesmus sp. Algal Res. 2015;11:22–7. https://doi.org/10.1016/j.algal.2015.05.020

(12) Přibyla P, Pilnýb J, Cepáka V, Kaštánekc P. The role of light and nitrogen in growth and carotenoid accumulation in Scenedesmus sp. Algal Res. 2016;16:69–75.https://doi.org/10.1016/j.algal.2016.02.028.

(13) Wang S, Stiles AR, Guo C, Liu C. Microalgae cultivation in photobioreactors: An overview of light characteristics. Eng Life Sci. 2014;14:550–9. https://doi.org/10.1002/elsc.201300170.

(14) Bohne F, Linden H. Regulation of carotenoid biosynthesis genes in response to light in Chlamydomonas reinhardtii. Biochim Biophys Acta. 2002;1579(1):26–34. https://doi.org/10.1016/S0167-4781(02)00500-6.

(15) Scharff C, Domurath N, Wensch-Dorendorf M, Schröder F. Effect of different photoperiods on the biochemical profile of the green algae C. vulgaris and S. obliquus. In: ISHS Acta Horticulturae 1170: International Symposium on New Technologies and Management for Greenhouses - GreenSys2015. 2017. p. 1149–56. https://doi.org/10.17660/ActaHortic.2017.1170.148.

1 2 3 > >> 
Received 2020-10-09
Accepted 2020-11-10
Published 2021-01-15