Contenido principal del artículo

En el presente estudio se estableció las condiciones para el cultivo autotrófico y mixotrófico de Scenedesmus sp, utilizando efluentes de drenajes ácidos de mina de carbón con el objeto de estudiar la producción de biomasa y de carotenoides tanto en condiciones autótrofas y mixotróficas en relación con el efecto de diferentes fuentes de nitrógeno: nitrato de sodio, la urea y el fosfato de amonio. Se determinó la remoción de contaminantes como DQO, COT, Metales pesados, iones y Sólidos Suspendidos totales. Los cultivos fueron por lote durante nueve días y se comparó la producción de biomasa, carotenoides y remoción de contaminantes en cada condición. La mayor concentración de biomasa alcanzada fue de 1,2 g/L se alcanzó en el cultivo mixotrófico para el medio con urea, seguido por del medio con nitrato de sodio de 0,9 g/L en una relación C/N de 6:1. La mayor concentración específica de carotenoides se alcanzó en el cultivo mixotrófico para el medio con nitrato de sodio y fue de 19.43 mg carotenoides / g biomasa. El medio con fosfato de amonio resultó ser inhibitorio para el crecimiento de la microalga con los resultados más bajos de todos los parámetros con excepción del cultivo autotrófico donde la concentración de carotenoides fue muy similar que las alcanzadas con el medio con nitrato de sodio y urea. Finalmente, el cultivo mixótrófico en el medio con urea presentó los mayores porcentajes de remoción siendo 86,97% para hierro total, 58,29% para cloruros y 92,5% para DQO.

1.
Urbina-Suárez NA. Efecto de diferentes fuentes de nitrógeno en el cultivo autotrófico y mixotrófico de Scenedesmus sp para la producción de biomasa y carotenoides utilizando efluentes ácidos de drenaje de minas de carbón. inycomp [Internet]. 30 de octubre de 2021 [citado 7 de diciembre de 2022];24(1). Disponible en: https://revistaingenieria.univalle.edu.co/index.php/ingenieria_y_competitividad/article/view/11284

(1) Jaimes-Duarte D-L, Soler-Mendoza W, Velasco-Mendoza J, Muñoz-Peñaloza Y, Urbina-Suárez N-A. Characterization chlorophytas microalgae with potential in the production of lipids for biofuels. CTyF - Ciencia, Tecnología y Futuro. 2012;5(1):93-102. https://doi.org/10.29047/01225383.210

(2) Hussain F, Shah SZ, Ahmad H, Abubshait SA, Abubshait HA, Laref A, et al. Microalgae an ecofriendly and sustainable wastewater treatment option: Biomass application in biofuel and bio-fertilizer production A review. Renew Sustain Energy Rev. 2021;137:110603. https://doi.org/10.1016/j.rser.2020.110603

(3) Solovchenko A, Verschoor AM, Jablonowski ND, Nedbal L. Phosphorus from wastewater to crops: An alternative path involving microalgae. Biotechnol Adv. 2016;34(5):550–64. http://dx.doi.org/10.1016/j.biotechadv.2016.01.002

(4) Manhaeghe D, Blomme T, Van Hulle SWH, Rousseau DPL. Experimental assessment and mathematical modelling of the growth of Chlorella vulgaris under photoautotrophic, heterotrophic and mixotrophic conditions. Water Res. 2020;184:116152. https://doi.org/10.1016/j.watres.2020.116152

(5) Vidotti ADS, Riaño-Pachón DM, Mattiello L, Giraldi LA, Winck F V., Franco TT. Analysis of autotrophic, mixotrophic and heterotrophic phenotypes in the microalgae Chlorella vulgaris using time-resolved proteomics and transcriptomics approaches. Algal Res. 2020;51:102060. https://doi.org/10.1016/j.algal.2020.102060

(6) Poddar N, Sen R, Martin GJO. Glycerol and nitrate utilisation by marine microalgae Nannochloropsis salina and Chlorella sp. and associated bacteria during mixotrophic and heterotrophic growth. Algal Res. 2018;33:298–309. https://doi.org/10.1016/j.algal.2018.06.002

(7) Kwon G, Le LT, Jeon J, Noh J, Jang Y, Kang D, et al. Effects of light and mass ratio of microalgae and nitrifiers on the rates of ammonia oxidation and nitrate production. Biochem Eng J. 2020;161:107656. https://doi.org/10.1016/j.bej.2020.107656

(8) Scarponi P, Volpi Ghirardini AM, Bravi M, Cavinato C. Evaluation of Chlorella vulgaris and Scenedesmus obliquus growth on pretreated organic solid waste digestate. Waste Manag. 2021;119:235–41. https://doi.org/10.1016/j.wasman.2020.09.047

(9) Song M, Pei H. The growth and lipid accumulation of Scenedesmus quadricauda during batch mixotrophic/heterotrophic cultivation using xylose as a carbon source. Bioresour Technol. 2018;263:525–31. https://doi.org/10.1016/j.biortech.2018.05.020

(10) Wang X, Zhang MM, Sun Z, Liu SF, Qin ZH, Mou JH, et al. Sustainable lipid and lutein production from Chlorella mixotrophic fermentation by food waste hydrolysate. J Hazard Mater. 2020;400(June):123258. https://doi.org/10.1016/j.jhazmat.2020.123258

(11) Ayre JM, Moheimani NR, Borowitzka MA. Growth of microalgae on undiluted anaerobic digestate of piggery effluent with high ammonium concentrations. Algal Res. 2017;24(Part A):218–26. https://doi.org/10.1016/j.algal.2017.03.023

(12) Moheimani NR, Webb JP, Borowitzka MA. Bioremediation and other potential applications of coccolithophorid algae: A review. Algal Res. 2012;1(2):120–33. https://doi.org/10.1016/j.algal.2012.06.002

(13) Cai T, Park SY, Li Y. Nutrient recovery from wastewater streams by microalgae: Status and prospects. Renew Sustain Energy Rev. 2013;19:360–9. https://doi.org/10.1016/j.rser.2012.11.030

(14) Goswami RK, Mehariya S, Verma P, Lavecchia R, Zuorro A. Microalgae-based biorefineries for sustainable resource recovery from wastewater. J Water Process Eng. 2021;40:101747. https://doi.org/10.1016/j.jwpe.2020.101747

(15) Feng X, Chen Y, Lv J, Han S, Tu R, Zhou X, et al. Enhanced lipid production by Chlorella pyrenoidosa through magnetic field pretreatment of wastewater and treatment of microalgae-wastewater culture solution: Magnetic field treatment modes and conditions. Bioresour Technol. 2020;306(March):123102. https://doi.org/10.1016/j.biortech.2020.123102

(16) Sánchez S, Martínez ME, Espejo MT, Pacheco R, Espinola F, Hodaifa G. Mixotrophic culture of Chlorella pyrenoidosa with olive-mill wastewater as the nutrient medium. J Appl Phycol. 2001;13(5):443–9. https://doi.org/10.1023/A:1011929723586

(17) Fu W, Wichuk K, Brynjólfsson S. Developing diatoms for value-added products: Challenges and opportunities. N Biotechnol. 2015;32(6):547–51. https://doi.org/10.1016/j.nbt.2015.03.016

(18) Kalra R, Gaur S, Goel M. Microalgae bioremediation: A perspective towards wastewater treatment along with industrial carotenoids production. J Water Process Eng. 2021;40:101794. https://doi.org/10.1016/j.jwpe.2020.101794

(19) Dickinson KE, Whitney CG, McGinn PJ. Nutrient remediation rates in municipal wastewater and their effect on biochemical composition of the microalga Scenedesmus sp. AMDD. Algal Res. 2013;2(2):127–34. http://dx.doi.org/10.1016/j.algal.2013.01.009

(20) Santana H, Cereijo CR, Teles VC, Nascimento RC, Fernandes MS, Brunale P, et al. Microalgae cultivation in sugarcane vinasse: Selection, growth and biochemical characterization. Bioresour Technol. 2017;228:133–40. http://dx.doi.org/10.1016/j.biortech.2016.12.075

(21) Caprio F Di, Altimari P, Iaquaniello G, Toro L, Pagnanelli F. T.obliquus mixotrophic cultivation in treated and untreated olive mill wastewater. Chem Eng Trans. 2018;64(May):625–30. https://doi.org/10.3303/CET1864105

(22) Torres DD, Cáceres Sepúlveda S, Roa AL, Suárez Gelvez JH, Urbina Suárez NA. Utilización de microalgas de la división Chlorophyta en el tratamiento biológico de drenajes ácidos de minas de carbón. Rev Colomb Biotecnol. 2017;19(2):95–104. https://doi.org/10.15446/rev.colomb.biote.v19n2.70429

(23) Cuéllar-García DJ, Rangel-Basto YA, Urbina-Suarez NA, Barajas-Solano AF, Muñoz-Peñaloza YA. Lipids production from Scenedesmus obliquus through carbon/nitrogen ratio optimization. Journal of Physics: Conference Series. 2019;1388:012043. https://doi.org/10.1088/1742-6596/1388/1/012043

(24) Wellburn AR. The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. J Plant Physiol. 1994;144(3):307–13. http://dx.doi.org/10.1016/S0176-1617(11)81192-2

(25) Wang J, Zhang S, He C, She Z, Pan X, Li Y, et al. Source identification and component characterization of dissolved organic matter in an acid mine drainage reservoir. Sci Total Environ. 2020; 739:139732. https://doi.org/10.1016/j.scitotenv.2020.139732

(26) Song C, Han X, Qiu Y, Liu Z, Li S, Kitamura Y. Microalgae carbon fixation integrated with organic matters recycling from soybean wastewater: Effect of pH on the performance of hybrid system. Chemosphere. 2020;248:126094. https://doi.org/10.1016/j.chemosphere.2020.126094

(27) Gallardo Martínez D, Bruguera Amarán N, Díaz Duque JA, Cabrera Díaz I. Drenaje ácido de minas y su influencia en ecosistemas asociados al yacimiento Santa Lucía, Cuba. Rev Iberoam Ambient Sustentabilidad. 2020;3(2):67–81. https://doi.org/10.46380/rias.v3i2.79

(28) Arumugam M, Agarwal A, Arya MC, Ahmed Z. Influence of nitrogen sources on biomass productivity of microalgae Scenedesmus bijugatus. Bioresour Technol. 2013;131:246–9. http://dx.doi.org/10.1016/j.biortech.2012.12.159

(29) Su Y. Revisiting carbon, nitrogen, and phosphorus metabolisms in microalgae for wastewater treatment. Sci Total Environ. 2021; 762:144590. https://doi.org/10.1016/j.scitotenv.2020.144590

(30) Qin L, Liu L, Wang Z, Chen W, Wei D. Efficient resource recycling from liquid digestate by microalgae-yeast mixed culture and the assessment of key gene transcription related to nitrogen assimilation in microalgae. Bioresour Technol. 2018;264(March):90–7. https://doi.org/10.1016/j.biortech.2018.05.061

(31) Lafarga T, Clemente I, Garcia-Vaquero M. Carotenoids from microalgae. In: Galanakis CM, editor. Carotenoids: Properties, Processing and Applications. Elsevier Inc.; 2020:149–187. Available from: https://doi.org/10.1016/B978-0-12-817067-0.00005-1

(32) Gong M, Bassi A. Carotenoids from microalgae: A review of recent developments. Biotechnol Adv. 2016;34(8):1396–412. http://dx.doi.org/10.1016/j.biotechadv.2016.10.005

(33) Park YT, Lee H, Yun HS, Song KG, Yeom SH, Choi J. Removal of metal from acid mine drainage using a hybrid system including a pipes inserted microalgae reactor. Bioresour Technol. 2013;150:242–8. http://dx.doi.org/10.1016/j.biortech.2013.09.136

(34) Krishna Samal DP, Sukla LB, Pattanaik A, Pradhan D. Role of microalgae in treatment of acid mine drainage and recovery of valuable metals. Mater Today Proc. 2020;30(Part 2):346–50. https://doi.org/10.1016/j.matpr.2020.02.165