3D computer model to estimate induced electrical parameters in an in-vitro culture stimulated by low-frequency magnetic fields

Abstract

Computational models are a continuous topic in research and development, which is used to support experimental procedures. These represent a fundamental tool to define increasingly specific variables to be applied by reducing costs and research time. In this study, a 3D computer model of human fibroblast and osteoblast cells stimulated by magnetic fields was developed. The model was created from the actual experimental scheme. It was constituted by the source (a Helmholtz coil), a FalconTM 96-well cell culture plate, and the cellular material. The electrical resistivity property of cells was measured in each material and the electrical resistivity property was assigned to the computer model. The applied magnetic flux density was 1 mT and 1,5 mT with frequencies between 15 Hz and 105 Hz. Electric field, current density, and electric heating were the variables evaluated. It was observed an exponential growth of induced signals with frequency and generated magnetic field level, this being more significant for the frequency.